Friday, October 18, 2019
Labour Turnover and Customer Service in Restaurants Essay
Labour Turnover and Customer Service in Restaurants - Essay Example As the discussion stresses restaurant, hotel and leisure industry is very much dependent on their employees for keeping their customer satisfied. This is a service industry where the intangibility, inseparability and heterogeneity of the service make it very challenging for the service provider to maintain consistency and increase the level of satisfaction received by the consumers.This discussion outlines that the labour turnover in the service industry is high. The high rate of turnover is result of various factors like lack of opportunities and growth, low income level and other reasons. The employees are the face of the service providing company. High employee turnover rate also indicates towards the management problems and internal issues of the organisation. Organisation is questioned for not able to retain its employees.Ã In this situation, it becomes very challenging for the human resource management professionals or business owner to ensure the stability of employees. Ther e are various strategies that a company adopts to attract and retain employees and maintain their image in the job market. Restaurant companies offer different types of monetary and non monetary benefits to its employees. The monetary benefits are incentives, bonus, stock options and others cash components.Ã The non monetary benefits include insurance, heath benefits, maternity and paternity leaves, education sponsorship, education assistance etc.
Thursday, October 17, 2019
Discrimination Of The African Americans Essay Example | Topics and Well Written Essays - 2250 words
Discrimination Of The African Americans - Essay Example However, the development in Southern part of America has been slow due to the presence of the ethnic group of African American. They are the people who faced dominance and low status in the nation due to their skin color. They have been deprived of various rights affecting their social and economic position1. The life of the people in this period has been was different and the amenities received depended a lot on their ethnicity and race. In this essay, the history and life of the African American are depicted who are facing challenges since 1877. In the year 1928, the life of the native people and the African Americans is dissimilar due to the presence of strong governance giving rise to inequality and a lack of self-esteem along with respect. In the current situation i.e. 1928, as an undergraduate student at Howard University, the perceived highs and lows of African Americans are being explored with the argument in between the period of 1877 to 1928. The African Americans live majo rly in the south and the prevailing challenging situation in the nation is affecting the lives of farmers. The jobs that are provided to the people of higher ranks are in the mines, tobacco, and ores. The blacks are deprived of every facility even if they have the potential than the whites. The educated blacks are positioned under the whites in order to suppress them. The position of the blacks although large in number is suppressed by the whites-only because of their color. Moreover, the women of the African American origin are forced to work for the whites as slaves. This is one of the low points faced by African Americans since 1877. This implies that according to the whites, the skin color is the only criteria for the people to get a job and status in the society irrespective of the fact the person has enormous potential2.
Assignment Example | Topics and Well Written Essays - 500 words - 139
Assignment Example In St. John, people hung out their clothes to dry as compared to Calgary where people use washers and dryers in their households (DeWolf , 2007). The use of clear examples and illustrations in the essay into drawing a conclusion about the topic makes it qualify as an inductive essay. According to the essay, the Christopher uses examples in different cities such as Calgary, St. John, Montreal, and St. Michael to demonstrate the significance of clotheslines. Apart from the aesthetic picture brought about by clothes hanging on the lines, there are a vast range of benefits associated with hanging clothes in the lines (DeWolf , 2007). According to La Presse, the sun is eradicates odours and removes stains from clothes in en effective manner as compared to dryers. There is a wide range of advantages of having clothesline apart from being fashionable, and aesthetic outstanding. The presence of clothesline in various regions brings out traditional aspect of hanging clothes in the open. According to research studies, the sun plays an imperative role in the elimination of odours and stains as compared to the use of dryer. Drying of clothes in the sun is sometimes associated with poverty and class. On this perspective, some governments have implemented legislations that ban individuals from hanging clothes in lines. On the contrary, Ontario environment minister has brought up the idea to eliminate clothesline ban in the region. Clotheslines have myriad benefits to people and the environment in general. According to Christopher, clotheslines have a beautiful and romantic outlook to the surrounding. During the authorââ¬â¢s visit at different cities, he observed various aesthetic outlook of clothesline. Clotheslines have traditional and more fashionable way of drying clothes in the open sun. On the other hand, the sun has a crucial role in the elimination of bad smell and stains on clothes. There have been myriad legislations
Wednesday, October 16, 2019
Discrimination Of The African Americans Essay Example | Topics and Well Written Essays - 2250 words
Discrimination Of The African Americans - Essay Example However, the development in Southern part of America has been slow due to the presence of the ethnic group of African American. They are the people who faced dominance and low status in the nation due to their skin color. They have been deprived of various rights affecting their social and economic position1. The life of the people in this period has been was different and the amenities received depended a lot on their ethnicity and race. In this essay, the history and life of the African American are depicted who are facing challenges since 1877. In the year 1928, the life of the native people and the African Americans is dissimilar due to the presence of strong governance giving rise to inequality and a lack of self-esteem along with respect. In the current situation i.e. 1928, as an undergraduate student at Howard University, the perceived highs and lows of African Americans are being explored with the argument in between the period of 1877 to 1928. The African Americans live majo rly in the south and the prevailing challenging situation in the nation is affecting the lives of farmers. The jobs that are provided to the people of higher ranks are in the mines, tobacco, and ores. The blacks are deprived of every facility even if they have the potential than the whites. The educated blacks are positioned under the whites in order to suppress them. The position of the blacks although large in number is suppressed by the whites-only because of their color. Moreover, the women of the African American origin are forced to work for the whites as slaves. This is one of the low points faced by African Americans since 1877. This implies that according to the whites, the skin color is the only criteria for the people to get a job and status in the society irrespective of the fact the person has enormous potential2.
Tuesday, October 15, 2019
Business Functions and Processes Essay Example | Topics and Well Written Essays - 5000 words
Business Functions and Processes - Essay Example Primary activities are mainly associated with the creation of a particular product, whereas support activities involve assignments that support the primary activities and each other. Three of the support activities that include procurement, human resource management and technology development can be connected to primary activities, while the fourth activity (firm infrastructure) is responsible for supporting the entire value chain. The concept of value chain postulated by Porter (1985) explains that attaining competitive advantage begins with the effort to build organizational proficiency in conducting specific competitive value chain activities. The underlying aim behind performing such activity is to attempt harnessing those capabilities, which strengthen competitiveness and strategy of an organization. Given the fact that one or more of these abilities become the cornerstone of a companyââ¬â¢s strategy and more resources are then allocated towards building greater expertise in performing these activities, the targeted abilities may ultimately become sustainable core competencies (Roper, Du and Love, 2008). In order to understand significance that Porter had attached to the value chain, it is imperative to recognize that there are two distinct and basic sources of competitive advantage. One source is a lower relative cost advantage and the other is a sort of differentiation. Although empirical research scholars have argued on the fact that many companies (specially Japanese) have been largely successful in combining the two sources mentioned above into one source, Porter continued to seek ways whereby both the sources could be developed separately (Hansen and Birkinshaw, 2007). Porterââ¬â¢s primary emphasis was on the argument that attaining low cost advantage or differentiation largely relies upon the disconnected activities performed by an organization. By disaggregating these activities in strategically pertinent groups, managers will be
Monday, October 14, 2019
South African Economic Racism Essay Example for Free
South African Economic Racism Essay Throughout the course of the 19th and 20th century, economic considerations played a significant role in shaping both racial discrimination and concepts of white supremacy in South Africa. When large deposits of gold and diamonds were discovered in the latter half of the 19th century, the economic structure of southern Africa was dramatically altered. The mining industry necessitated vast amounts of inexpensive labor in order to be profitable. In the post South African War period, the necessity to return the Boers to self-rule without harming the mining industry resulted in a series of legislation that legalized racial discrimination to ensure a large and cheap labor force consisting of Africans. South Africaââ¬â¢s industrial revolution and the legislation passed during the period immediately following the South African War provided the economic bases of racial discrimination and white supremacy in South Africa. In 1868, the Kimberly diamond strike propelled South Africa to a position as a world leader in diamond production. Similarly, the discovery of large gold deposits in the 18th and 19th century also attracted both foreign capital investment and immigration. The rapidly growing mining industries required massive amounts of inexpensive labor in order to be profitable. Hundreds of thousands of African workers sought employment in the developing mines and cities in industrializing cities. In the face of this increased competition, white workers benefitted from racial discrimination because it protected them economically from competition with African workers. White business owners sought a large and cheap African workforce; racial discriminatory practices ensured they were cheap and pliable. In order to limit the bargaining power of African workers seeking higher wages and to ensure that none could flee in the face of harsh labor conditions, the British conquered the remaining independent African states, confiscated land, and imposed severe cash taxations during the 1880s and 1890s. This forced African labor that had previously chosen employment in the mining industry voluntarily to now do so under conditions set solely by the employers. In addition, a series of discriminatory practices were first introduced during this period including pass laws and urban ghettos, further ensuring the enforced cooperation and steady supply of cheap African labor. The industrial revolution in South Africa introduced a pressing need for incredible amounts of cheap labor. The introduction of raciallyà discriminatory practices sought to meet this need by ensuring the In the aftermath of the South African War in 1902, the mining industry saw a depression in the face of a severe labor short age. In response, South African capitalists and mining industry leaders worked with British imperial authorities to import massive amounts of Chinese laborers. Between 1902 and 1907, over one hundred thousand Chinese immigrated to South Africa. However, Afrikaners opposed this vehemently arguing that it should be a white manââ¬â¢s country and that this influx of labor represented a new competitive threat for potential jobs. In 1906, the Liberals come to power in Great Britain. Many had opposed the South African War and were now calling for the return of self-rule to the Boers without endangering the mining industry. Afrikaners would take the place of skilled and semi-skilled English laborers at lower wages while newly enacted policies would force greater numbers of Africans to unskilled labor. The 1911 Mines and Works Act restricted African employment to menial and unskilled jobs, excluding them from most skilled categories of work. In effect, it legislated a racial hierarchy in the workplace and extended the practice throughout the South African economy beyond the mining industry. Furthermore, the Industrial Conciliation Act of 1924 restricted African rights to organize or to negotiate their terms of employment. In 1913, the Nativesââ¬â¢ Land Act was enacted, restricting African ownership of land to designated areas comprising roughly 7 percent of the South Africaââ¬â¢s total land area and restricting Africans from establishing any businesses outside of this allocated space. In addition, the land partitioned for Africans was often of poor quality and could not possibly support its population. Thus, the law effectively prevented Africans from leading self-sufficient lives in rural communities. They were increasingly forced to work on Afrikaner owned farms, factories, and mines. In addition, restrictions on their movement and the right to reside in urban areas combined with the inability for rural areas to support significant numbers of Africans resulted in an endless system of migrant unskilled labor. Economic interests and considerations significantly in fluenced the segregation policies adopted in South Africa during the 19th and 20th centuries. The necessity of a cheap and controllable labor source resulted in harsh racially discriminatory policies and the rise of white supremacy. In 1944, the chairman of the Broederbond stressed the special role ofà Afrikaners in a speech, ââ¬Å"In every people in the world is embodied a Divine Idea and the task of each People is to build upon that Idea and to perfect it. So God created the Afrikaner People with a unique language, a unique philosophy of life, and their own history and tradition in order that they might fulfill a particular calling and destiny here in the southern corner of Africa. We must believe that God has called us to be servants of his righteousness in this placeâ⬠(Clark and Worger, 32). Placing emphasis on divine support for their existence, their special place in the world, and righteous mission, this Afrikaner ideology can draw historical antecedents from the belief of Manifest Destiny in the United States in the 19th century and from the ethnic nationalist movement of Nazi Germany in the mid 20th century. This emphasis on the special Afrikaner identity heavily influenced Hendrik Verwoerd who se argument for apartheid centered on the inability for two cultures to achieve their ideal futures as each of their paths are unique. The intermingling of languages, traditions, and histories would be detrimental to both the Afrikaner and African peoples. These ideals of Afrikaner nationalism expressed in the quote draws historical antecedents back into the 19th century concept of Manifest Destiny. During the 19th century, Manifest Destiny was a widely held belief in the United States that American settlers were destined to expand throughout the North American continent due to the special virtues of the American people and through a special destiny that was divinely ordained. Similarly, the Broederbond chairman asserts the special role of the Afrikaner people and their divinely ordained origin. In addition, he argues for their expansion throughout southern Africa as both a divine calling and righteous mission. The ideas expressed in the quote by the Broederbond chairman also draw numerous parallels with the 20th century German nationalism found in Nazi Germany. Following his ascension to power in Germany, Hitlerââ¬â¢s movement and theories resonated considerably in South Africa, particularly with the Purified National Party and the Broederbond. The Broederbond established a ââ¬Å"voortrekkerâ⬠movement for Afrikaner youth, arguably seen as analogous to the Hitler Youth . In 1938, the Ossewabrandwag was established on the model of Hitlerââ¬â¢s national socialist movement. By the end of 1939, over three hundred thousand Afrikaners had sworn loyalty to the volk, or Afrikaner people. It sought to establish an Afrikaner controlled republic in Southà Africa, by violent means if necessary. Additionally, in 1938, a centenary celebration of the Great Trek was organized that placed emphasis on the heroic struggle of the Afrikaners against British oppression. The articulation of this ââ¬Å"sacred historyâ⬠and of a struggle for survival sought to memorialize and legitimize the special place of the Afrikaner people. These sentiments are again arguably analogous to those found in the ethnic nationalist movement of Nazi Germany that stressed the special place in history of the Aryan race. In both Afrikaner nationalism and German nationalism, the nation has a mystical and divine quality. In addition, the Afrikaner and German people are also considered to possess certain special and unique characteristics such as language, culture, and history that transcend the individual and contribute to a national unity. The emphasis of a special and unique Afrikaner identity expressed in the quote by the Broederbond chairman in 1944 can be seen in Hendrik Verwoerdââ¬â¢s argument for apartheid in 1950. As the minister of native affairs in the first National Party government, Verwoerd met with African members of the Native Representative Council to explain his theory and justification of apartheid. Basing his argument in the concept of ethnic nationalism, he asserts that Afrikaner and African communities must be divorced in order for both parties to prosper. It is impossible for two distinct and unique peoples to achieve their ideal futures while intermingled because each has their own particular path and calling. Verwoerd argues that tension and conflict are inevitable in mixed societies. The mixing of languages, traditions, and cultures in South Africa are only detrimental to the development of both the Afrikaner and African people. Appealing to African leaders for their cooperation in installing an apartheid policy, Verwoerd states that, ââ¬Å"Instead of striving after vague chimeras and trying to equal the European in an intermingled community with confused ideals and inevitable conflict, he can be a national figure helping to lead his own people along the road of peace and prosperity. He can help give the educated men and women of his people an opportunity toâ⬠¦fully realize their ambitions within their own sphereâ⬠(Clark and Worger, 141). His explanation of apartheid places a significant emphasis on the purity of a peopleââ¬â¢s descent and the continuation of this line as the key to secure an ideal future. Both Afrikaners and Africans can only truly realize their full potential within their own cultural and ethnic sphere.
Sunday, October 13, 2019
Purification of Fructosyl Transferase (FTase)
Purification of Fructosyl Transferase (FTase) Introduction Food products are formulated for taste, appearance, cost and convenience of the consumer. The composition of food products that confer a health benefit is a relatively new trend, and recognizes the growing acceptance of the role of diet in disease prevention, treatment and well-being. It is thus becoming increasingly clear that there is a strong relationship between the food we consume and our health. Scientific knowledge of the beneficial role of various food ingredients (nutrients) for the prevention of specific diseases is rapidly advancing. Functional foods, pharma foods and nutraceuticals are synonyms for foods that can prevent and treat diseases. Generally, a functional food can be defined as ââ¬Ëany food that has a positive impact on an individualââ¬â¢s health, physical performance or state of mind in addition to its nutritional contentââ¬â¢. Functional foods in addition to their basic nutritional content and natural being, will contain the proper balance of ingredients which will help to improve many aspects of human lives, including the prevention and treatment of illness and disease (Goldberg, 1994). So far, a large number of functional foods in various forms have already been introduced into the market. Many of them contain a number of characteristic functional ingredients. They include dietary fiber, oligosaccharides, sugar alcohols, peptides and proteins, prebiotics and probiotics, phytochemicals, antioxidants and polyunsaturated fatty acids (Stark and Madar, 1994). Oligosaccharides are very well recognized as ââ¬Ëfunctional food ingredientsââ¬â¢ because of their positive effects on human health. This research work focuses on the microbial production and purification of Fructosyl Transferase (FTase) and the production of Fructooligosaccharides (FOS) by transfructosylation using this enzyme. Oligosaccharides Oligosaccharides are usually defined as carbohydrates with a low degree of polymerization (DP) and consequently low molecular weight. They are composed of 3-10 simple sugar units linked together.The main categories of nondigestible oligosaccharides include carbohydrates in which the monosaccharide unit is fructose, galactose, glucose and xylose. Chemical differences among commercially available oligosaccharides include chain length, monosaccharide composition, degree of branching and purity. They are found naturally (small amounts) in many plants.. Plants with large amounts of oligosaccharides includeà chicory root, from which most commercial inulin is extracted, and so-called Jerusalem. They are also found in onions,à leeks, garlic, legumes, wheat, asparagus, jicama, and other plant foods. Oligosaccharides are functional food ingredients that have great potential to improve the quality of many foods. In addition to providing useful modifications to food flavor and physicochemical characteristics, they possess properties that are beneficial to the health of the consumers. In general, food grade oligosaccharides are not pure products, but are mixtures containing oligosaccharides of different degrees of polymerization, the parent polysaccharide or disaccharide and the monomer sugars (Roberfroid and Slavin, 2000). The production and application of food grade oligosaccharides are increasing and their major uses are in beverages, infant milk powders, confectioneries, bakery products, yoghurts and dairy desserts. FRUCTOOLIGOSACCHARIDES FOS are chain polymers of the sugar fructose that are found in a variety of foods. The sugar units can be linked in a single straight chain or can be branched. In many cases small amounts of glucose are also contained in the chain. Chemically, FOS is difficult to define, because the length of the fructose chains can vary from source to source. Therefore a FOS analysis of a food may report the total of several closely related compounds. Inulin is an example of a longer chained compound that is considered as FOS. The shorter (lower molecular weight) compounds tend to have a sweet taste. The size and complexity of the FOS molecule desirable characteristics. Although the simple sugars fructose and glucose are quickly absorbed into the body by the intestines, FOS for the most part is indigestible and therefore acts as a non-digestible fiber in the diet. This is because humans does not have the enzymes to break down FOS as it travels down the digestive tract. When the FOS reaches the large intestine and the colon, the bacteria that are found there start to break down the FOS. These bacteria have the enzymes needed to break down FOS. Bifidobacteria have been reported to use FOS. It is believed that foods that promote bifidobacterial growth are good for the health. Of all the oligosaccharides known so far, FOS has attracted special attention. FOS is a common name for fructose oligomers that are mainly composed of 1- Kestose (GF2), 1 Nystose (GF3) and 1F ââ¬â Fructofuranosyl nystose (GF4) in which fructosyl units (F) are bound at the à ² 2, 1 position of sucrose (GF). Structure of Fructooligosaccharides Occurrence FOS derived from sucrose occur in many higher plants as reserve carbohydrates. They are found in a variety of edible plants, including banana, barley, garlic, honey, onion, rye, brown sugar, tomato, asparagus root, Jerusalem artichoke, wheat and triticale (Fishbein et al, 1988). The concentration of FOS in these foods is diagrammatically represented in Figure 1.2 (derived using data from the Environmental Protection Agencyââ¬â¢s Dietary Risk Evaluation system EPA, 1984). FOS like 1- kestose, neokestose, 6- kestose and their derivatives have also been isolated from the plant Agave vera cruz (Satyanarayana, 1976). FTase producing organisms FTase by transfructosylation activity helps in the production of FOS. The enzyme source of FOS synthesis can be divided into two classes; one is plants such as asparagus, sugar beet, onion, Jerusalem artichoke etc; the other consists of bacteria and fungi such as Arthrobacter sp., Fusarium sp., Aspergillus sp., Aureobasidium sp. Functional properties of FOS Low calorific value The à ²- configuration of the anomeric carbon, C2 in their fructose monomers make FOS nondigestible by human digestive enzymes which are mostly specific for à ±-osidic linkages and hence they are not utilized as an energy source in the body. However, due to colonic fermentation, they have an energy contribution to food of about 1.5 kcal/ g. This property makes them suitable for use in sweet, low-calorie diet foods and safe for consumption by individuals with diabetes. In the case of very sweet foods, they may be used as bulking agents in conjunction with intense artificial sweeteners such as aspartame, phenylalanine or sucralose, thereby masking the aftertaste produced by some of these intense sweeteners (Crittenden and Playne, 1996). Non cariogenicity Unlike starch and simple sugars, FOSs are not utilized by oral microflora like Streptococcus mutans to form acids and insoluble à ²-glucans, that serve as a matrix for plaque formation and are the main culprits in causing dental caries (Oku, 1994). Hence, FOSs are presently used as non ââ¬â cariogenic sugar substitutes in confectionery, chewing gums, yoghurts and drinks. Dietary fibre effect ââ¬ËDietary fibre is the edible part of plants or analogous carbohydrates that are resistant to digestion and absorption in the human small intestine with complete or partial fermentation in the large intestineââ¬â¢ (AACC Report, 2001). The nondigestible quality of FOS makes them act similar to dietary fibre, and thus prevent constipation. However, excessive consumption of FOS may cause flatulence or diarrhea, the minimum dose being 40-50 g/day. The advantages FOS has over dietary fibre are that they have a smaller daily requirement, do not cause diarrhea in recommended doses, are slightly sweet, have neither bad texture nor bad taste, are completely water soluble, do not build viscosity, do not bind minerals, are physically stable, and are easier to incorporate into processed foods and drinks (Tomomatsu H, 1994). Prebiotic effect In recent years, the ability of FOS to promote the proliferation of bifidobacteria in the colon has been recognized. These intestinal bacteria metabolize FOS readily and produce large amounts of short chain fatty acids resulting in an acidic pH in the lumen of the large intestine. The beneficial bacteria such as Bifidobacterium sp. and Lactobacillus sp. are resistant to the acidic pH, whereas the harmful bacteria such as Clostridium sp. are sensitive to the acidic conditions. Therefore the proliferation of useful bacteria is stimulated and that of harmful bacteria is suppressed. Subsequently, FOS has been described as one of several prebiotics, which can be defined as ââ¬Ëa nondigestible food ingredient that beneficially affects the host by selectively stimulating the growth and / or the activity of one or a limited number of bacteria in the colon and thus improves host healthââ¬â¢ (Gibson and Roberfroid, 1995). FOS have been demonstrated to increase bifidobacteria numbers in the colon at doses of Lipid lowering effect FOS in diet has been found to decrease the levels of triglycerides, serum cholesterol and lipids. Hypotriglyceridemia is due to decrease in the hepatic synthesis of triglycerides and hypocholesterolemia is likely to result from the antagonistic effect of short chain fatty acids, especially propionate on cholesterol metabolism. Propionate has been found to be an inhibitor of HMG CoA reductase, an important enzyme in cholesterol synthesis (Roberfroid and Slavin, 2000). Effect on mineral absorption FOS have been found to enhance the absorption of Ca2+ and Mg2+ ions and maintain balance of iron and Zn2+ ions. This has been found to be due to the osmotic effect, acidification of the colonic content due to fermentation and production of short chain carboxylic acids, formation of calcium and magnesium salts of these acids and hypertrophy of the colon wall (Roberfroid and Slavin, 2000). Anticancer effect FOS has an indirect effect on prevention of cancer in human beings due to its prebiotic properties. This is due to immunity enhancements by the cells, cell wall components and extracellular components of bifidobacteria (Tomomatsu H, 1994). Production of nutrients The presence of a good colonic environment with high bifidobacterial count leads to the production of Vitamins B-1, B-2, B-6, B-12, nicotinic acid and folic acid. Bifidobacteria ââ¬â fermented dairy products also improve lactose tolerance, calcium absorbability and digestibility (Tomomatsu H, 1994). Antidiabetic effect FOS have been claimed to have no effect on blood glucose levels in patients with type 2 diabetes, possibly due to effects of short chain fatty acids produced during fermentation. (Luo et al, 2000). PROBLEM STATEMENT Normally, pure sucrose is expensive and the cost to produce FTase by using sucrose is high. There are some by-product like cereal bran, corn-products, sugarcane molasses and by-products of coffee and tea processing industries were used as a substrate to produce FTase from microorganism (Sangeetha et al., 2003). This by-product can lower the cost of production of FTase. Before FOS can be produced, the enzyme FTase must be undergone a series of purification. The purification of FTase must be done in order to get high yield of FOS. Many researchers have reported the purification and characterization of FTase from various sources and FTase has been found to differ in their molecular weight and properties from one source to another (Lateef et al., 2006, Sangeetha et al., 2003). FTase exhibits hydrolytic activity which can dominate the process (Delphine et al., 2007). This fact will lead to lower production yields and to a contamination of the final product with glucose and fructose (Delphine et al., 2007). Purification of FTase is important for batch production of FOS. By doing so, the nature of its hydrolytic activity can be studied, improve the understanding its mode of operation and be able to classified which type of enzyme should it belong to (Là ¢Ã¢â ¬Ã
¸Hocine et al., 2000). RESEARCH OBJECTIVES The main objective of this research is to purify FTase enzyme from microfungi Aspergillus oryzae isolated from CFTRI. To enhance the production of FOS as a food additive. SIGNIFICANCE OF STUDY In Malaysia, the FTase enzyme used to be imported from other country such as Japan, India and United States (Sangeetha et al., 2005). Normally, the cost for production of FOS from commercialized enzyme is high, so by producing and purifying the enzyme, we can reduce the cost. Normally, crude enzyme will produce lower yield of FOS than purified enzyme. In order to fulfill high customer demand on the functional food such as FOS, the production of FOS must be increased. In order to produce high yield of FOS, the enzyme need to be purified. By doing this, the activity of enzyme will be increased and so do the production of FOS. Purification and characterization of the enzyme (FTase) are necessary steps to obtain the product (FOS) rapidly, with high purity, to improve our understanding of its mode of action and the nature of activity. Efforts have been made to purify the FTase enzyme to get high specific activity and fold of purification but the reaction mechanism involved in FOS production does not lead to higher yields than the theoretical maxima of 56 ââ¬â 58 % due to inhibition caused by accumulation of glucose. Nevertheless, the time involved in obtaining maximum FOS yield has been considerably reduced by using the purified FTase in comparison to the crude FTase. The product FOS formed has also been characterized for its physicochemical properties and structure using HPLC or LC-MS. Studies on the prebiotic effects of FOS are required to establish its functional properties and to demonstrate its use in product development. The present research work has also focused on the scaling up of both the produ ction of FTase as well as FOS.
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